Manipulation of Tree Node Objects¶
Section author: Tony Walters
Examples of how to initialize and manipulate various tree node objects.
>>> from cogent.core.tree import PhyloNode >>> from cogent import LoadTree >>> from cogent.parse.tree import DndParser
The general method to initialize a tree is
LoadTree, however, for exceptionally large trees or if one needs to specify the node objects (
DndParser should be used.
PhyloNode objects by default.
The basic properties of the tree node objects are:
TreeNodeobjects are general purpose in nature, and lack phylogenetic distance values.
PhyloNodeobjects inherit the methods of the
TreeNodeclass and in addition contain phylogenetic distances.
RangeNodeobjects contain evolution simulation methods in addition to the standard features of a
The following demonstrates the two methods for initializing a phylogenetic tree object.
>>> simple_tree_string="(B:0.2,(C:0.3,D:0.4)E:0.5)F;" >>> complex_tree_string="(((363564 AB294167.1 Alkalibacterium putridalgicola:0.0028006,55874 AB083411.1 Marinilactibacillus psychrotolerans:0.0022089):0.40998,(15050 Y10772.1 Facklamia hominis:0.32304,(132509 AY707780.1 Aerococcus viridans:0.58815,((143063 AY879307.1 Abiotrophia defectiva:0.5807,83619 AB042060.1 Bacillus schlegelii:0.23569):0.03586,169722 AB275483.1 Fibrobacter succinogenes:0.38272):0.06516):0.03492):0.14265):0.63594,(3589 M62687.1 Fibrobacter intestinalis:0.65866,314063 CP001146.1 Dictyoglomus thermophilum:0.38791):0.32147,276579 EU652053.1 Thermus scotoductus:0.57336);" >>> simple_tree=LoadTree(treestring=simple_tree_string) >>> complex_tree=DndParser(complex_tree_string, PhyloNode)
Now to displaying, creating, deleting, and inserting a node in simple_tree. Note that simple_tree has three tips, one internal node ‘E’, and the root ‘F.’ For this example, we will create a node named ‘A’, with a distance of 0.1, delete the node ‘C’ through its parent, the internal node ‘E’, and finally we will insert ‘A’ where ‘C’ once was.
Display the original tree.
>>> print simple_tree.asciiArt() /-B -F-------| | /-C \E-------| \-D
Create a new node object.
Display the children of the root node, one of which is the parent of the tip we wish to alter. To add or remove a node, we need to use the parent of the target node, which in this case is the internal node ‘E.’
>>> print simple_tree.Children [Tree("B;"), Tree("(C,D)E;")]
Remove the ‘C’ tip. Note:
removeNode() return ‘True’ if a node is removed, ‘False’ if they cannot remove a node.
>>> simple_tree.Children.remove('C') True
Insert the new ‘A’ tip where ‘C’ was previously.
Finally, display the modified tree.
>>> print simple_tree.asciiArt() /-B -F-------| | /-A \E-------| \-D
When deleting tree nodes, it is often desirable to clean up any unbranched internal nodes that may have resulted from removal of tips. For example, if we wanted to delete the node ‘A’ that was previously added, the resulting tree would have an unbranched internal node ‘E.’
>>> simple_tree.Children.remove('A') True >>> print simple_tree.asciiArt() /-B -F-------| \E------- /-D
prune() method, internal nodes with only a single branch are removed.
>>> simple_tree.prune() >>> print simple_tree.asciiArt() /-B -F-------| \-D
An Example of Conditional Tree Node Modifications¶
Now to look at the more complex and realistic tree. In complex_tree, there are no internal nodes or a defined root. In order to display this tree in a more succinct manner, we can rename these tips to only contain the genus and species names. To step through the tips only, we can use the
iterTips() iterator, and rename each node. The
asciiArt() function, by default, will attempt to display internal nodes; this can be suppressed by the parameter
First, let’s split the ungainly name string for each tip and only preserve the genus and species component, separated by a space.
>>> for n in complex_tree.iterTips(): ... n.Name=n.Name.split()+" "+n.Name.split()
Now we display the tree with
>>> print complex_tree.asciiArt(show_internal=False) /-Alkalibacterium putridalgicola /--------| | \-Marinilactibacillus psychrotolerans /--------| | | /-Facklamia hominis | | | | \--------| /-Aerococcus viridans | | | | \--------| /-Abiotrophia defectiva | | /--------| ---------| \--------| \-Bacillus schlegelii | | | \-Fibrobacter succinogenes | | /-Fibrobacter intestinalis |---------| | \-Dictyoglomus thermophilum | \-Thermus scotoductus
For another example of manipulating a phylogenetic tree, let us suppose that we want to remove any species in the tree that are not closely related to Aerococcus viridans. To do this, we will delete any nodes that have a greater phylogenetic distance than 1.8 from Aerococcus viridans. The best method to remove a large number of nodes from a tree is to first create a list of nodes to delete, followed by the actual removal process. It is important that the
prune() function be called after deletion of each node to ensure that internal nodes whose tips are deleted are removed instead of becoming tips. Alternatively, one could test for internal nodes whose children are deleted in the procedure and flag these nodes to be deleted as well.
First, generate a list of tip nodes.
Next, iterate through this list, compare the distances to Aerococcus, and append to the deletion list if greater than 1.8.
>>> tips_to_delete= >>> AEROCOCCUS_INDEX=3 >>> for n in tips: ... if tips[AEROCOCCUS_INDEX].distance(n)>1.8: ... tips_to_delete.append(n)
Now for the actual deletion process. We can simply use the parent of each node in the deletion list to remove itself. Pruning is necessary to prevent internal nodes from being left as tips. Note:
removeNode() return ‘True’ if a node is successfully removed, ‘False’ otherwise.
>>> for n in tips_to_delete: ... n.Parent.remove(n) ... complex_tree.prune() True True True
Finally, print the modified complex_tree.
>>> print complex_tree.asciiArt(show_internal=False) /-Alkalibacterium putridalgicola /--------| | \-Marinilactibacillus psychrotolerans --------- /--------| | /-Facklamia hominis | | \--------| /-Aerococcus viridans | | \--------| /-Abiotrophia defectiva | /--------| \--------| \-Bacillus schlegelii | \-Fibrobacter succinogenes